ad-gfp  (Vector Biolabs)

Journal: bioRxiv

Article Title: Progenitor Diversity and Architecture of the Human Ganglionic Eminences Shaping the Basal Ganglia

doi: 10.64898/2025.12.31.697063

Figure Lengend Snippet: A) Experimental scheme of organoid culture. B) Example immunostaining of H28126 line at DIV25. Scale bar is 500mm. C) Quantification using Imaris software of NKX2.1 protein expression at DIV21 (3 cell lines). D) ScRNA-seq analysis of MGE organoids with UMAP clustering revealing 29 distinct cell clusters based on principal component analysis (PCA). E) Proportion of MST divisions relative to non-MST divisions in organoids at DIV25 and DIV47 (3 cell lines). F) Average MST translocation length. G) Immunostaining of organoid slice following live imaging and infection with Ad-CMV-GFP.

Article Snippet: Slices were incubated floating in cell culture medium according to age of organoids with 1:1000 Ad-CMV-GFP (vectorbiolabs; #1060) at 37°C for 3-5 days.

Techniques: Immunostaining, Software, Expressing, Translocation Assay, Imaging, Infection

Journal: Biochemistry and Biophysics Reports

Article Title: LIN28-mediated gene regulatory loops synchronize transitions throughout organogenesis

doi: 10.1016/j.bbrep.2025.102226

Figure Lengend Snippet: Transcriptional regulation of LIN28A and LIN28B by developmental transcription factors. A) Schematic of transcription factors that orchestrate germ layer formation and early embryonic lineage specification and differentiation. B) Top: Schematic of morphogen-induced lung/endoderm formation from iPSCs through initiation of lung progenitors in vitro. Bottom: Gene expression analysis of single cell RNA-sequencing of roughly 10,000 cells (close 1200 for each day) throughout morphogen-directed lung endoderm formation from iPSCs. Data from manuscript (Ori et al., 2023). C) Fold change in gene expression of LIN28A and LIN28B upon infection of embryonic lung fibroblast WI-38, with adenoviral constructs: GFP, FOXA2, NKX2-1, SOX2 and SOX9 for 48–72 h before lysing in Trizol. Dots on the graph representative of n = 2 independent experiments in quadruplicate. D) Schematic of development of endoderm differentiation. Transient transfection of txrn factors into HEK293 with construct stably expressing dual luciferase construct; firefly and Renilla luciferase. Validation of a subset of factors that regulated human LIN28A and LIN28B promoter/enhancer sequences during screening experiments in panels B) and C) during E) pluripotency and F) endoderm/lung development. G) progenitor specification. Graphs in E), F) and G) are representative of n = 2 independent validation experiments out of 3. P values for C) were determined using 2-way ANOVA of normal distribution. P values of significance: In the order of left to right for panel C: LIN28A – FOXA2- ∗0.024, NKX2-1- ∗0.035, SOX9- ∗0.021; LIN28B – NKX2-1- ∗0.021, ∗SOX9- 0.021 P values for graphs E), F) and G) were determined using 1-way ANOVA of normal distribution in E) and 1-way ANOVA of lognormal distribution for F) and G). P values of significance: In the order of left to right for each panel: E)∗ 0.0375, ∗∗ 0.0021; ∗ 0.0184, ∗0.0321 F)∗ 0.0434, ∗0.0325; ∗0.0191, ∗0.0477 G)∗∗0.0097,∗ 0.0112; ∗∗0.0021, ∗∗0.0078; ∗∗ 0.0036, ∗∗0.0079; ∗∗0.0072, ∗0.0160. Schematics A), B), D) were made using BioRender.

Article Snippet: Lin28a/b were knocked out post-neurosphere formation via adenoviral infection with Ad-Cre-GFP adenovirus (Vector Biolabs, 1700) or Ad-GFP adenovirus (Vector Biolabs, 1060).

Techniques: In Vitro, Gene Expression, RNA Sequencing, Infection, Construct, Transfection, Stable Transfection, Expressing, Luciferase, Biomarker Discovery

Journal: Journal of Molecular and Cellular Cardiology Plus

Article Title: Rab10 plays a protective role in the development of pathological cardiac hypertrophy

doi: 10.1016/j.jmccpl.2025.100494

Figure Lengend Snippet: Rab10 overexpression inhibited Ang II-induced cardiomyocyte hypertrophy and Ang II-initiated signaling pathway. (A) Western blot analysis of exogenous Rab10 in NRCMs infected with Ad-GFP or Ad-Rab10 with anti-Myc or anti-Rab10 antibody. Quantification of the relative Rab10 level (lower, n = 3, three independent replicates). (B) Representative images of NRCMs infected with Ad-GFP or Ad-Rab10 and then treated with 200 nmol/L Ang II for 24 h. Hypertrophy was assessed by cell surface area measurements ( n = 3, 150 cells counted per experiment; right). (C) qPCR analysis of the ANP mRNA level in NRCMs ( n = 3, three independent replicates). (D) Representative immunoblotting analysis of p-ERK1/2, ERK1/2, p-AKT and AKT in NRCMs infected with Ad-GFP or Ad-Rab10 and treated with 200 nmol/L Ang II ( n = 3, three independent replicates). (E) Quantification of the relative protein levels (right). β-actin was used as a loading control. Data are presented as mean ± s.e.m., and n represents number of samples. * P < 0.05 , ** P < 0.01 versus Ad-GFP or saline; # P < 0.05, ## P < 0.01 versus Ad-GFP plus Ang II.

Article Snippet: Recombinant adenoviruses expressing the green fluorescent protein (GFP) alone (Ad-GFP), the full-length Rab10 cDNA with a C-terminal Myc-tag (Ad-Rab10), driven by the cytomegalovirus promoter were generated using AdEasy (MP Biomedicals).

Techniques: Over Expression, Western Blot, Infection, Control, Saline